Hplc Capillary Column Device

ABSTRACT

A high pressure liquid chromatography (HPLC) capillary column device, system and method for processing a HPLC sample with a cartridge housing a packed capillary column; at least one inlet connection to the capillary column for a sample fluid; and at least one outlet connection from the capillary column for the sample fluid. The outlet connection is able to accommodate either a spray tip for atomizing the sample fluid or a transport tube for transporting the sample fluid from a spray tip column to a spray tip. Inlet connections enable supply of electrical power to the capillary column through electrical connections disposed within the cartridge housing; and gas for evaporating the sample liquid is supplied to at least one outlet connection from the capillary column for the sample fluid through a gas supply line within the cartridge housing. The temperature of the sample liquid can be controlled through a heat connection.

CROSS REFERENCE RELATED APPLICATION INFORMATION

This application claims priority from U.S. Provisional Patent Application No. 60/60/507,725 filed Oct. 1, 2003. The contents of these applications are incorporated herein by reference.

BACKGROUND OF THE INVENTION

Capillary columns used for high pressure liquid chromatography (HPLC) are fragile and difficult to manipulate. A high level of care must be taken when handling and connecting the capillary to an HPLC system for analysis by a mass spectrometer. This is typically a tedious and time consuming process. Also, temperature control of the capillary column is generally difficult to establish.

SUMMARY OF THE INVENTION

It is an object of this invention to provide a HPLC system for interfacing between a chromatographic system and a mass spectrometer

It is an object of this invention to provide a HPLC capillary column device of reduced size which also reduces the risk of damage and reduces handling time via a cassette cartridge arrangement.

It is an object of this invention to provide a HPLC capillary column device which enables accurate temperature control and heating capabilities.

The present invention is directed to a high pressure liquid chromatography (HPLC) capillary column device for processing a HPLC sample. The device has a cartridge housing, and the cartridge housing comprises: a packed capillary column inside the cartridge housing; at least one inlet connection to the capillary column for a sample fluid; and at least one outlet connection from the capillary column for the sample fluid. The outlet connection is able to accommodate one of a spray tip for atomizing the sample fluid and a transport tube for transporting the sample fluid from a spray tip column to a spray tip. An inlet connection enables supply of electrical power to the capillary column through electrical connections disposed within the cartridge housing; and an inlet connection enables supply of a gas to the at least one outlet connection from the capillary column for the sample fluid through a gas supply line disposed within the cartridge housing, with the gas supply line being fluidically coupled to the outlet connection.

The HPLC capillary column device can further comprise a heat connection enabling a heat supply to be coupled to the capillary column and the heat supply can control the temperature of the HPLC device during the HPLC processing. The heat supply can be either electrical or a heated fluid. Voltage applied to the sample liquid by the electrical power supply ionizes at least a portion of the sample liquid.

The invention can also be in the form of a cartridge for a high pressure liquid chromatography (HPLC) capillary column device for processing a HPLC sample, with the device comprising a cartridge housing as described above.

The housing can have a multi-sided cross-section and the capillary column can be disposed in a straight line from the inlet connection to the outlet connection, or alternatively, in a curvilinear line from the inlet connection to the outlet connection.

The housing can have a substantially curvilinear cross-section which can further comprise at least one portion that is not uniformly disposed with respect to the substantially curvilinear cross-section. The portion that is not uniformly disposed is capable of acting as an alignment key for the cartridge to be inserted into a mass spectrometer interface.

The present invention can be in the form of a high pressure liquid chromatography (HPLC) system for interfacing between a chromatographic system and a mass spectrometer. The HPLC system comprises: a HPLC capillary column cartridge cassette, the cartridge cassette including connections for controlling gas flow, temperature and voltage applied to a liquid sample within the capillary column. The cartridge cassette is capable of receiving a sample and chromatographic data from the chromatographic system. The system further comprises a mass spectrometer interface device, with the mass spectrometer interface device including a cartridge cassette holder for holding the HPLC capillary cartridge cassette. The cartridge cassette holder is capable of transmitting the liquid sample in a charged atomized condition to the mass spectrometer. In addition, the gas flow, temperature and voltage of the cartridge cassette are capable of being controlled by the mass spectrometer.

The present invention also provides a method of processing a sample from a HPLC capillary column device with the HPLC capillary column device comprising a cartridge for a packed capillary column. The method is performed by: choosing an appropriate cartridge for one of a procedure being conducted and a compound being detected; inserting the cartridge into a mass spectrometer interface; connecting a connection on the cartridge with a gas supply line supplied to the sample liquid through the gas supply line for evaporating at least a portion of the sample liquid; evaporating at least a portion of the sample liquid; connecting a connection on the cartridge with an electrical power supply line for atomizing the sample liquid at a liquid junction of the packed capillary column; atomizing the sample liquid at a liquid junction of the packed capillary column; and establishing flow from a chromatographic system and to a mass spectrometer through the mass spectrometer interface by injecting a sample liquid into the chromatographic system;

Furthermore, the method can also comprise the steps of maintaining temperature of the capillary column in a range of 5 to 70° C. above ambient temperature; and analyzing, by the mass spectrometer, flow emerging as a spray from a stage of the mass spectrometer interface. A spray tip can be employed for the spray to the mass spectrometer. A transport tube can be employed for transporting the spray to the mass spectrometer, with the transport tube being either rigid or flexible.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a schematic view of the HPLC system of the present invention.

FIG. 2A is a plan view of an HPLC capillary column device according to a first embodiment of the present invention having a spray tip.

FIG. 2B is a plan view of an HPLC capillary column device according to an alternate arrangement of the first embodiment of the present invention having a transport tube to a spray tip.

FIG. 3 is a plan view of an HPLC capillary column device according to a second embodiment of the present invention having a spray tip.

FIG. 4A is a plan view of an HPLC capillary column device according to a third embodiment of the present invention having a spray tip.

FIG. 4B is an end view of the HPLC capillary column device according to the third embodiment of the present invention according to FIG. 4A having a spray tip.

FIG. 4C is a perspective view of the HPLC capillary column device according to the third embodiment of the present invention of FIGS. 4A and 4B.

FIG. 5A is a plan view of an HPLC capillary column device according to an alternate arrangement of the third embodiment of the present invention having a transport tube to a spray tip.

FIG. 5B is an end view of the HPLC capillary column device according to the alternate arrangement of the third embodiment of the present invention according to FIG. 5A having a transport tube to a spray tip.

FIG. 5C is a perspective view of the HPLC capillary column device according to the alternate arrangement of the third embodiment of the present invention of FIGS. 5A and 5B.

FIG. 6A is a plan view of a fourth embodiment of the present invention of FIG. 4 or 5A.

FIG. 6B is an end view of the HPLC capillary column device according to the alternate arrangement of the third embodiment of the present invention according to FIG. 6A having a transport tube to a spray tip.

FIG. 6C is a perspective view of the HPLC capillary column device according to the alternate arrangement of the third embodiment of the present invention of FIGS. 6A and 6B.

DETAILED DESCRIPTION OF EXEMPLARY EMBODIMENTS

The present invention provides an HPLC capillary column device which facilitates the interface of the device with a mass spectrometer. To facilitate the description of the present invention, the features of the prior art are described as follows.

FIG. 1 is a schematic view of the HPLC system of the present invention. HPLC capillary column cassette 200 is provided to facilitate the interface of the electrospray mass spectrometry unit. In particular, a spray tip, voltage source and connections 24 and 26, and the desolvation gas flow are now readily interfaced with a mass spectrometer 340 having a front side 260. The chromatographic system 230 provides the chromatographic spectra for a sample (not shown). The chromatographic spectra are output through a connection 235 to the HPLC column cassette 200. The HPLC column cassette is then inserted into a cassette holder 250 of mass spectrometer interface device 240. The mass spectrometer interface device 240 interfaces by means of a spray 255 of the sample which emerges from stage 252 which travels to the front side 260 of mass spectrometer 340. Typically, the mass spectrometer 340 controls the parameters such as the temperature, desolvation gas flow and the applied voltage for the HPLC column cassette 200 but control can also be exerted from the mass spectrometer interface device 240 or from the chromatographic system 230. Also, although the mass spectrometer interface device 240 is illustrated in FIG. 1 as being separate from the chromatographic system 230 and from the mass spectrometer 340, the interface device 240 can also be integral with either the chromatographic system 230 or the mass spectrometer 340. In addition, the HPLC column cassette 200 can be designed into several different specific embodiments as follows.

First Embodiment

FIG. 2A is a plan view of an HPLC capillary column device according to the first embodiment of the present invention. The HPLC pump and injector injects a liquid sample through an inlet connection 202 contained within the rear side surface 204 of a cassette cartridge 200-1. The capillary column 160 is disposed within the cassette cartridge 200. The capillary column is packed in a manner commonly applied by those skilled in the art. A heat source connection 206, a voltage source connection 212, and a gas source connection 216 are each contained within the front side surface 220 of the cassette cartridge 200-1.

Heat provided through the column heat source connection 206, sufficient to maintain the temperature of the HPLC device in a range typically of 5 to 70° C. above ambient temperature, is conducted through a flow tube or channel 208 disposed internally within the cassette cartridge 200-1. The heat source connection 206 is fluidically coupled, when the heat source is a heated fluid, or electrically coupled, when the heat source is an electrical resistance wire, to the flow tube or channel 208 that surrounds the capillary column 160 within the cassette cartridge 200-1 to provide a means for accurate temperature control and heating of the sample fluid within the capillary column 160.

The voltage provided through the voltage source connection 212, typically in the range of 4 KV DC, is directed by the voltage connections 241 and 261 to atomize and disperse the liquid sample emerging from the outlet 224 of the capillary column 160 that is within the conductive adapter 222 into a very fine spray of electrically charged droplets. The spray tip 280 immediately downstream of the capillary column outlet 224 within the conductive adapter 222 directs the sample spray 32 towards the mass spectrometer 340.

The gas, typically but not limited to nitrogen, provided through the gas source connection 216 again provides desolvation gas flow at pressures and temperatures sufficient to increase the rate of evaporation of the solvent of the liquid sample during the electrospray ionization (ESI) process occurring as a result of the voltage applied through the voltage connections 241 and 261. The gas source connection 216 is fluidically coupled to gas flow tube or channel 218 internally disposed within the cassette cartridge 200-1, the gas flow tube or channel 218 in turn being fluidically coupled to the spray tip 280 through the conductive collar 222.

FIG. 2B is an alternate arrangement of the first embodiment disclosed in FIG. 2A. The only difference is that instead of spray tip 280 being immediately downstream of capillary column 160, the transport tube 360 is immediately downstream of capillary column 160. In all other respects, the cassette cartridge 200 is identical to that disclosed in FIGS. 1A and 1B. The application of the voltage for atomization at the voltage connections 241 and 261 occurs at the liquid junction 380-1. The gas evaporates the liquid sample solvent within the conductive adapter 222.

Second Embodiment

FIG. 3 is a plan view of a second embodiment of the present invention where cassette cartridge 200-2 contains the same components as illustrated in FIG. 2A for cassette cartridge 200-1, including the spray tip 280, but instead of a straight capillary column, such as capillary column 160 in FIGS. 2A and 2B, a coiled capillary column 162 is provided instead within what is typically a circular enclosure 270 of radius R. In all other respects, the coiled capillary column cassette cartridge 200-2 is the same as cassette cartridge 200-1. Furthermore, those skilled in the art recognize that while the coiled capillary column cassette cartridge 200-2 is shown with a spray tip configuration as in FIG. 2A, the transport tube configuration described in FIG. 2B can be applied to the coiled column cartridge 200-2 as well.

Third Embodiment

FIG. 4A is a plan view of the third embodiment of the present invention. The third embodiment differs from the first and second embodiments in that a tubular cartridge rather than a flat cartridge houses the capillary column. Specifically, the HPLC pump and injector 12 injects the liquid sample into inlet connection 802 of tubular cassette cartridge 804. The tubular cassette cartridge 804 encloses the capillary column 806 typically along the centerline of the tubular cartridge 804, which typically is comprised of, but is not limited to, a circular cross-section. A column heat block 808 in the tubular cassette cartridge 804 provides insulation of heat flow to the capillary column 806 that is provided through heat source connection 812. The heat source connection 812 is formed on the outer surface of the tubular cassette cartridge 804 and, depending on whether the heat source is a heated fluid or an electrical resistance source, fluidically or electrically couples tube or channel 814 to the capillary column 806.

Further downstream, at the outlet end of the tubular cassette cartridge 804, a conductive adapter 818 surrounds a spray tip 828. A voltage source connection 822 penetrates the conductive adapter 818 by way of voltage connections 824 and 826. The voltage provided through the voltage source connection 822, again typically in the range of 4 KV DC, is directed by the voltage connections 824 and 826 to atomize and disperse the liquid sample 14 emerging from the end of the capillary column 806 at the liquid junction interface 838 that is within the conductive adapter 818 into a very fine spray of electrically charged droplets. The spray tip 820 immediately downstream of the end of the capillary column 806 within the conductive collar 818 directs the sample spray 32 towards the mass spectrometer 240, which in this case interfaces with the tubular cassette cartridge 804 by way of an interfacing port 844 contained within a cassette holder 850 that is contained within the mass spectrometer 240. In this case, the cassette holder 850 is designed to facilitate the interconnection with the now tubular-shaped cartridge 804, as discussed below for FIGS. 4B and 4C.

As before with respect to the first and second embodiments, the gas, typically but not limited to nitrogen, provided through the gas source connection 834 again provides desolvation gas flow at pressures and temperatures sufficient to increase the rate of solvent evaporation during the electrospray ionization (ESI) process. The gas source connection 834 is fluidically coupled to gas flow tube or channel 836 internally disposed within the tubular cassette cartridge 804 and which in turn is fluidically coupled to the spray tip 820 through the conductive adapter 818.

FIG. 4B is an elevation view of the inlet end of the tubular cassette cartridge 804 of FIG. 5A. The alignment key 840 is provided to facilitate proper insertion of the tubular cartridge 804 into the cassette holder 850 within the mass spectrometer interface 240.

FIG. 4C is a perspective view of the tubular cassette cartridge 804 of FIG. 4A showing the inlet connection 806, the heat source connection 812, the voltage source connection 822, the gas source connection 834, and the spray tip 820.

FIG. 5A is a plan view of an alternate arrangement of the third embodiment of the present invention illustrated in FIG. 4A. The only difference is that instead of spray tip 820 being immediately downstream of capillary column 806, the transport tube 842 is immediately downstream of capillary column 806. As is the case for the transport tube 360 of FIG. 2B, the transport tube enables transport of the atomized charged droplets to a mass spectrometer that is a distance away from the chromatographic system 230. In all other respects, the tubular cassette cartridge 804 is identical to that disclosed in FIG. 7A. As a result, FIG. 5B is identical to FIG. 4B. The only difference between FIG. 5C and FIG. 4C is the presence of the transport tube 842 instead of the spray tip 820.

Fourth Embodiment

FIG. 6A is a plan view of a fourth embodiment of the present invention of FIG. 4A or 5A. In this case, the tubular cassette cartridge 804 of FIG. 4A or of FIG. 5A incorporates, instead of a straight capillary column 806, a coiled or helical capillary column 856 within the column heat block 808 Either a spray tip 820 or a transport tube to a spray tip 842 can be employed as is obvious to those skilled in the art. In all other respects, the tubular cassette cartridge 804, as shown in FIGS. 6A through 6C can be constructed and used in a similar manner as shown in FIGS. 4A-4C and FIGS. 5A-5C.

With respect to the method of using the first, second, third and fourth embodiments, referring to FIG. 1, the user chooses an appropriate cartridge, shown generically as cartridge cassette 200 to represent cartridge cassettes 200-1, 200-2 or 804 of FIGS. 2A through 6C as appropriate, for the procedure being conducted or the compound being detected. The user inserts either cartridge 200-1, 200-2 or 804 into the mass spectrometer interface 240 and makes the connections to the respective gas line 216 or 834, the electrical power for the voltage connection 212 or 822 and from the temperature control mechanism as represented by column heat connections 206 or 812. The user establishes flow from the chromatographic system 230 and to the mass spectrometer 340 through the mass spectrometer interface 240. The sample is injected into the chromatographic system 230 and emerges from the stage 252 as a spray 255 to be analyzed by the mass spectrometer 340 to obtain the desired results. When using a cartridge with a transport tube, such as transport tube 360 in FIG. 3B, or transport tube 842 in FIGS. 5A and 5C or the cartridge 200-2 in FIG. 3 when equipped with a transport tube, the transport tube can be either rigid or flexible. The design of the stage 252 can be such that either a rigid or flexible transport tube can be accommodated, as is obvious to those skilled in the art.

The invention has now been explained with reference to specific embodiments. Other embodiments will be apparent to those of ordinary skill in the art in view of the foregoing description. It is not intended that this invention be limited except as indicated by the appended claims and their full scope equivalents. 

1. A high pressure liquid chromatography (HPLC) capillary column device for processing a HPLC sample, said device having a cartridge housing, said cartridge housing comprising: a packed capillary column inside said cartridge housing; at least one inlet connection to said capillary column for a sample fluid; at least one outlet connection from said capillary column for the sample fluid, said outlet connection able to accommodate one of a spray tip for atomizing the sample fluid and a transport tube for transporting the sample fluid from a spray tip column to a spray tip; an inlet connection for supplying electrical power to said capillary column through electrical connections disposed within said cartridge housing; and an inlet connection for supplying a gas to the at least one outlet connection from said capillary column for the sample fluid through a gas supply line disposed within said cartridge housing, said gas supply line fluidically coupled to said outlet connection.
 2. The HPLC capillary column device according to claim 1 further comprising a heat connection enabling a heat supply to be coupled to said capillary column.
 3. The HPLC capillary column device according to claim 2, wherein said heat supply controls the temperature of the HPLC device during the HPLC processing.
 4. The HPLC capillary column device according to claim 3, wherein said heat supply maintains a temperature range of 5 to 70° C. above ambient temperature.
 5. The HPLC capillary column device according to claim 2, wherein said heat supply is one of (a) electrical and (b) a heated fluid.
 6. The HPLC capillary column device according to claim 1, wherein voltage applied to the sample liquid by the electrical power supply ionizes at least a portion of the sample liquid.
 7. The HPLC capillary column device according to claim 1, wherein a gas supplied to the sample liquid through the gas supply line evaporates at least a portion of the sample liquid.
 8. The HPLC capillary column device according to claim 1 wherein said housing has a multi-sided cross-section.
 9. The HPLC capillary column device according to claim 1 wherein said capillary column is disposed in a straight line from said inlet connection to said outlet connection.
 10. The HPLC capillary column device according to claim 1 wherein said capillary column is disposed in a curvilinear line from said inlet connection to said outlet connection.
 11. The HPLC capillary column device according to claim 1 wherein said housing has a substantially curvilinear cross-section.
 12. The HPLC capillary column device according to claim 11 wherein said substantially curvilinear cross-section further comprises at least one portion that is not uniformly disposed with respect to said substantially curvilinear cross-section.
 13. The cartridge according to claim 12 wherein said at least one portion that is not uniformly disposed is capable of acting as an alignment key for said cartridge to be inserted into a mass spectrometer interface.
 14. A high pressure liquid chromatography (HPLC) system for interfacing between a chromatographic system and a mass spectrometer, said HPLC system comprising: a HPLC capillary column cartridge cassette, said cartridge cassette including connections for controlling gas flow, temperature and voltage applied to a liquid sample within said capillary column, said cartridge cassette capable of receiving chromatographic data from the chromatographic system; and a mass spectrometer interface device, said mass spectrometer interface device including a cartridge cassette holder for holding said HPLC capillary cartridge cassette, said cartridge cassette holder capable of transmitting the liquid sample in a charged atomized condition to the mass spectrometer.
 15. The high pressure liquid chromatography (HPLC) system according to claim 14, wherein the gas flow, temperature and voltage of the cartridge cassette are capable of being controlled by the mass spectrometer.
 16. The high pressure liquid chromatography (HPLC) system according to claim 14, wherein said mass spectrometer interface device is integral with one of the mass spectrometer and the chromatographic system.
 17. A cartridge for a high pressure liquid chromatography (HPLC) capillary column device for processing a HPLC sample, said device comprising: a cartridge housing; a packed capillary column inside said cartridge housing; at least one inlet connection to said capillary column for a sample fluid; at least one outlet connection from said capillary column for the sample fluid, said outlet connection able to accommodate one of a spray tip for atomizing the sample fluid and a transport tube for transporting the sample fluid from a spray tip column to a spray tip; an inlet connection for supplying electrical power to said capillary column through electrical connections disposed within said cartridge housing; and an inlet connection for supplying a gas to the at least one outlet connection from said capillary column for the sample fluid through a gas supply line disposed within said cartridge housing, said gas supply line fluidically coupled to said outlet connection.
 18. The cartridge according to claim 17 further comprising a heat connection enabling a heat supply to be coupled to said capillary column.
 19. The cartridge according to claim 18, wherein said heat supply controls the temperature of the HPLC device during the HPLC processing.
 20. The cartridge according to claim 19, wherein said heat supply maintains a temperature range of 5 to 70° C. above ambient temperature.
 21. The HPLC capillary column device according to claim 18, wherein said heat supply is one of (a) electrical and (b) a heated fluid.
 22. The cartridge according to claim 17, wherein voltage applied to the sample liquid by the electrical power supply ionizes at least a portion of the sample liquid.
 23. The cartridge according to claim 17, wherein a gas supplied to the sample liquid through the gas supply line evaporates at least a portion of the sample liquid.
 24. The cartridge according to claim 17 wherein said housing has a multi-sided cross-section.
 25. The cartridge according to claim 17 wherein said capillary column is disposed in a straight line from said inlet connection to said outlet connection.
 26. The cartridge according to claim 17 wherein said capillary column is disposed in a curvilinear line from said inlet connection to said outlet connection.
 27. The cartridge according to claim 17 wherein said housing has a substantially curvilinear cross-section.
 28. The cartridge according to claim 27 wherein said substantially curvilinear cross-section further comprises at least one portion that is not uniformly disposed with respect to said substantially curvilinear cross-section.
 29. The cartridge according to claim 28 wherein said at least one portion that is not uniformly disposed is capable of acting as an alignment key for said cartridge to be inserted into a mass spectrometer interface.
 30. A method of processing a sample from a HPLC capillary column device, the HPLC capillary column device comprising a cartridge for a packed capillary column, the method performed by: choosing an appropriate cartridge for one of a procedure being conducted and a compound being detected; inserting said cartridge into a mass spectrometer interface; connecting a connection on said cartridge with a gas supply line supplied to the sample liquid through the gas supply line for evaporating at least a portion of the sample liquid; evaporating at least a portion of the sample liquid; connecting a connection on said cartridge with an electrical power supply line for atomizing said sample liquid at a liquid junction of said packed capillary column; atomizing said sample liquid at a liquid junction of said packed capillary column; and establishing flow from a chromatographic system and to a mass spectrometer through the mass spectrometer interface by injecting a sample liquid into the chromatographic system.
 31. The method of claim 30 further comprising the step of maintaining temperature of the capillary column in a range of 5 to 70° C. above ambient temperature.
 32. The method of claim 30 further comprising the step of analyzing, by the mass spectrometer, flow emerging as a spray from a stage of said mass spectrometer interface.
 33. The method of claim 30 wherein a spray tip is employed for the spray to said mass spectrometer.
 34. The method of claim 30 wherein a transport tube is employed for said spray to said mass spectrometer.
 35. The method of claim 34 wherein said transport tube is rigid.
 36. The method of claim 34 wherein said transport tube is flexible. 